Journal: Frontiers in Immunology

Article Title: Dynamics of necroptosis in kidney ischemia-reperfusion injury

doi: 10.3389/fimmu.2023.1251452

Figure Lengend Snippet: Comparison of cleaved Caspase-3 staining in WT and Mlkl -ko mice at 24 hrs post-reperfusion. (A) : WT; (B) : Mlkl -ko; (C) : sham; (D) : semi-quantitative analysis. Mann Whitney U test with * p<0.05 compared to sham procedure and # p<0.05 compared to WT mice. Data presented as mean ± SEM. Image magnification x 200.

Article Snippet: Sections were then incubated with rabbit monoclonal anti-phospho-MLKL (pSer345) (ab196436, 1:100, Abcam, Melbourne, Australia) or rabbit monoclonal anti-human/mouse cleaved Caspase-3 (cC3) (Asp175) (IC835G, 1:200, RD Systems, Noble Park, Australia) in 2% swine serum at 4°C overnight.

Techniques: Comparison, Staining, MANN-WHITNEY

Journal: Molecular Therapy Oncology

Article Title: Trabectedin decreases myeloid resistance to improve the efficacy of anti-PD1 immunotherapy and delay glioma malignant progression

doi: 10.1016/j.omton.2026.201147

Figure Lengend Snippet: Combination trabectedin and anti-PD1 ICB prolongs survival of glioma-bearing mice (A) Schematic depicting the treatment regimen for the Ntv-a x XFM-luc RCAS murine glioma progression model. Four groups of mice were treated with vehicle, trabectedin (2 doses, 1 week apart, starting at week 4), anti-PD-1 immune checkpoint blockade (ICB; 3 doses, 3 days apart, starting at week 3.5), or a combination of trabectedin and anti-PD-1 ICB. (B) Kaplan-Meier survival analysis of the four treatment groups. Vehicle ( n = 12), anti-PD1 ( n = 8), trabectedin ( n = 13), and trabectedin + anti-PD1 ( n = 12) had median survivals of 39, 43, 43, and 53 days, respectively. Significance was determined using the log-rank (Mantel-Cox) test ( p < 0.0001∗∗∗∗, p < 0.0002 ∗∗∗, p < 0.0021∗∗, p < 0.0332∗). (C) Representative images of cleaved caspase-3 (c-cas3) immunohistochemical (IHC) staining of tumors isolated from glioma-bearing mice 3 days post treatment 2 (3DPT2) with vehicle, trabectedin, or trabectedin + anti-PD1 combination. (D) Quantification of c-cas3 IHC staining. Positive pixels were calculated by sampling 5 equal-sized regions within each tumor core and comparing the number of positive pixels per region ( n = 2–5 mice per treatment group). Significance was determined using a paired t test with Welch’s correction ( p < 0.0005∗∗∗∗, p < 0.005 ∗∗∗, p < 0.005∗∗, p < 0.05∗). Error bars show SD.

Article Snippet: Slides were washed in TBST and stained with c-cas3 (R&D Systems MAB835, Minneapolis, MN) at a 1:100 dilution for 2 h at room temperature.

Techniques: Immunohistochemical staining, Immunohistochemistry, Isolation, Sampling